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Name: Saleh Mahmood, Ph. D.
Professor
Department of Biochemistry and Molecular Biology
University of Dhaka
Dhaka-1000, Bangladesh
Phone: 880-2-9354636
Email: salehmahmood14226@yahoo.com mahmoods@bangla.net

Academic Qualification

1994: Ph. D. (Biochemistry & Molecular Biology), Osaka University, Japan.
Ph. D Thesis: organizations and Characteristics of the control regions of genes for H+/K+-ATPase beta subunit.
Supervisor: Professor M. Futai. 1990: Post Graduate Diploma (Microbiology), Osaka University, Japan.
1986: MSc. : (Biochemistry), University of Dhaka, Bangladesh.
1983: BSc. : (Biochemistry), University of Dhaka, Bangladesh.

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Professional Experience

1. 2001-present, Associate Professor, Dept of Biochemistry & Molecular Biology, University of Dhaka, Bangladesh.

2. 1998-2001, Postdoctoral Research Associate, Department of Biochemistry, Faculty of Medicine and Biomedical Sciences, State University of New York at Buffalo, USA.

3. 1998-2001, Associate Professor, (on leave), Department of Biochemistry, University of Dhaka, Bangladesh.

4. 1994-1998, Assistant Professor, Assistant Professor, Department of Biochemistry, University of Dhaka, Bangladesh.

5. 1995, Postdoctoral Trainee, GBF, Braunchweig, Germany.

6. 1987-1994, Lecturer, Department of Biochemistry, University of Dhaka, Bangladesh.

7. 1986-1987, Research Associate, International Center for Diarrhoeal Disease Research, Bangladesh, (ICDDR,B)

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Honors and Fellowships

1. 1998-2001: Postdoctoral fellowship supported by NIH grants
2. 1995: German National Biotechnology (Fellowship)
3. 1991-1994: Japanese government (Monbusho) fellowship
4. 1989-1990: UNESCO fellowship
5. 1983: Undergraduate award

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Professional Society Membership

1.Life Member, Bangladesh Society for Biochemistry and Molecular Biology (BSBMB)
2. Executive Member: Bangladesh Society for Microbiologists

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Experience in Molecular Biological Techniques

Purification: genomic DNA, total RNA and plasmid DNA
cloning: genomic cloning, cloning of transcription factor gene by yeast one-hybrid system, cloning by PCR, prokaryotic transformation, labeling and detection of nucleic acids.
PCR and RT-PCR: amplification of DNA, amplification of RNA, site-directed mutagenesis, genotyping.

Electrophoresis: detection of DNA by Southern blot, detection of RNA by Northern blot, detection of DNA-protein interaction by gel-shift assay and DNase foot printing assay, sequencing of nucleic acids.

Cell culture and expression: maintenance of mammalian and prokaryotic cell culture, stable and transient transfection, CAT assays, detection and analysis of expressed proteins.

Gene knock-outs: Maintenance of gene-targeted animals, identification and detection of targeted gene, analysis of knock-out embryos.

Molecular Biology tools: gene data base research, gene homology search, DNA to amino acid search, prediction of promoter region, prediction of open reading frame, motif search, search for transcription factor binding sites, restriction enzyme site mapping.

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Technical activities

1. Course taught on molecular biology, genetics, general biochemistry, biochemistry of metabolism, human physiology and endocrinology, general microbiology, microbial metabolism and industrial biotechnology at undergraduate and masters level.

2. Supervision of thesis students at masters level.

3. Service on dissertation and oral examination committees in biochemistry.

4. Conducted biochemistry lab at masters level.

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Other experience

Attended National and International seminar and symposium and presented papers

List of Publications (Selected)

1. Johnson, M. T., Mahmood, S., Hyatt, S. L., Yang, H. S., Soloway, P. D., Hanson, R. W., and Patel, M. S. (2001) Inactivation of the murine pyruvate dehydrogenase (Pdha 1) gene and its effects on early embroyonic development. Mol. Genet. Metabol. 74, 293-302.

2. Patel, M. S., Johnson, M. T., Hyatt, S., Mahmood, S., H. S. yang, Soloway, P.Hanson, R. W. (2000) Targeted disruption of the murine Pdha 1 gene. FASEB J. 14, A1427 (Abstract 664).

3. Yoshida, T., Sato, R., Saleh Mahmood, Kawasaki, S., Futai, M., and Maeda, M. (1997)GATA-6 DNA binding protein expressed in human gastric adenocarcinoma MKN45 cells. FEBS Lett. 414, 333-337.

4. Ketudat-Cairns, M., Esipov, R, Saleh Mahmood and Zamrod, Z. (1995) Computer assisted analysis of cis-regulated elements in T-cell specific genes.GBF, Braunchweig, Germany.

5. Saleh Mahmood (1994) Organization and characterization of control regions of genes for H+/K+- ATPase beta subunit and intrinsic factor. A Doctoral Thesis, Faculty of Science, Osaka University, Japan.

6. Maeda, M., Oshiman, K., Tamura, S., Kaya, S., Saleh Mahmood, Reuben, M. A., Lasater, L. S., Sachs, G. and Futai, M. (1991) The rat H+/K+- ATPase beta subunit gene and recognition of its control region by gastric DNA binding Protein. J. Biol. Chem. 266, 21584-21588

7. Oshiman, K., Motojima, K., Saleh mahmood, Shaimda, A., Tamura, S., Maeda, M. and Futai, M. (1999) Control region and gastric specific transcription of the rat H+/K+- ATPase alpha subunit gene. FEBS Lett. 281, 251-254.

8. Maeda, M., Tamura, S. Oshiman, K., Saleh MAhmood, Amakatsu, K., Ishizaki, J. and Futai, M. (1990) Structural Studies of gastric H+/K+- ATPase. International Symposium of Proton pump. PP. 43-46.

9. Saleh Mahmood, Iwamoto, A., Maeda, M. and Futai, M. (1990) Suppression of uncD mutaion of Escherichia coli H+- ATPase (FOF1). ICBIOTECH 13, 89-94.

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Copyright© Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.