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Mailing
Address
M. Nazmul Huda,
Ph. D
Department of Biochemistry and Molecular Biology,
University of Dhaka, Dhaka-1000, Bangladesh.
Email: mnhuda308@yahoo.com
Personal Details:
Date of Birth: 31st December, 1966
Place of Birth: Sherpur, Bangladesh
Academic
qualification
Ph.D.: Okayama University, Japan (2002)
Dissertation: Molecular genetic analysis and biochemical characterization
of multidrug efflux pumps in Vibrio cholerae non-01.
Diploma: The 25th International University UNESCO Post-graduate
Course in Microbiology, Osaka & Sendai, Japan from October 1997
to September 1998.
M.Sc.(Thesis): Department of Biochemistry, University of Dhaka,
Bangladesh (1988).
B.Sc.(Hons): Department of Biochemistry, University of Dhaka,
Bangladesh (1987).
Training
Advanced laboratory training course in infectious disease research.
8-23 September 2003. Organized by ICDDR,B-Howard
Hughes Medical Institut
Teaching Experience:
July,1997-date:
Assistant Professor, Department of Biochemistry and Molecular Biology,
University of Dhaka, Bangladesh.
May,1993-June1997: Lecturer, Department of Biochemistry and Molecular
Biology, University of Dhaka, Bangladesh.
August, 2002-date: Part-time teacher, Department of Genetic Engineering
and Biotechnology, University of Dhaka, Bangladesh.
Research
Interests
Antibiotic resistance in the community is a growing threat particularly
in developing countries. Bacteria can be resistant in their natural
state or they can acquire resistance genes by mutation, expression
of a latent chromosomal gene or by exchange of genetic material from
other bacteria. They are a number of mechanisms by which bacteria
perform their resistant activity. Among them, multidrug efflux pump
is one of the common and major mechanisms. I have cloned and characterized
three multidrug efflux genes from Vibrio cholerae non-01. Two
of them belongs to the MATE family and the rest one belong to
ABC-type multidrug transporter family. I like to find some inhibitor(s)
from the natural sources that would be a potent inhibitor of the efflux
pumps.
Seminar and
Symposium attended:
1) Characterization
of an ABC type multidrug efflux pump in Vibrio cholerae non-01. Huda,
M.N., Morita, Y. Kuroda, T., Mizushima, T., and Tsuchiya, T. October
2001. The 54th Pharmaceutical conference (Chugoku region)
2) Cloning and characterization of a multidrug efflux pump
from Enterococcus faecalis. Lee, E.-W. Huda, M.N., Morita, Y. Kuroda,
T., Mizushima, T., and Tsuchiya, T. October 2001. The Annual Meeting
of the Japanese Biochemical Society.
3) Cloning of an ABC-type multidrug efflux pump from Vibrio
cholerae non-01. Huda, M.N., Morita, Y. Kuroda, T., Mizushima, T.,
and Tsuchiya, T. April, 2001. The 74th Annual Meeting of Japanese
Bacteriological Society.
4) Development of assay systems for multidrug efflux activity
using fluorescent antimicrobial drugs. Chen, J., Huda, M.N., Morita,
Y. Kuroda, T., Mizushima, T., and Tsuchiya, T. April, 2001. The 74th
Annual Meeting of Japanese Bacteriological Society.
5) Characterization of an Na+-driven multidrug efflux pump,
VmrA from Vibrio cholerae non-01. Huda, M.N., Morita, Y., Mizushima,
T., and Tsuchiya, T. October 2000. The 39th Annual conference of Japanese
Pharmaceutical Society and Japanese Hospital Pharmacist Society (Chugoku
region)
6) Multidrug efflux pumps in Vibrios. Morita, Y., Huda, M.N.,
Chen, J., Hayashi, Mizushima, T., and Tsuchiya, T. The 34th Vibrio
Symposium, Japan.
7) Cloning and characterization of an Na+-driven multidrug
efflux pump from Vibrio cholerae non-01. November, 1999. Huda, M.N.,
Morita, Y. Kuroda, T., Mizushima, T., and Tsuchiya, T. The 33th Vibrio
Symposium, Okinawa, Japan.
Selected
Publications
1)
Lee E-W, Huda M.N., Kuroda T, Mizushima T, Tsuchiya T. 2003.
EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis. Antimicrob
Agents Chemother. 47(12): 3733-8.
2) Huda, M.N., Lee, E-W., Chen, J., Kuroda, T., Mizushima,
T., and Tsuchiya, T. 2003. Molecular Cloning and Characterization
of an ABC-Type Multidrug Efflux Pump VcaM from Vibrio cholerae non-O1.
Antimicrob. Agents Chemother. 47(8):2413-7.
3) Chen J., Kuroda T., Huda M.N., Mizushima T., Tsuchiya T.
2003.An RND-type multidrug efflux pump SdeXY from Serratia marcescens.
J. Antimicrob. Chemother., 52: 308-311.
4) Huda, M. N., Chen. J., Morita. Y., Kuroda, T., Mizushima,
T., and Tsuchiya.T. 2003. Gene cloning and characterization of VcrM,
a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1.
Microbiol. Immunol., 47(6): 419-427
5) Lee, E-W., Chen, J. Huda, M. N., Kuroda, T., Mizushima,
T., and Tsuchiya, T. 2003. Functional cloning and expression of emeA,
and characterization of EmeA, a multidrug efflux pump from Enterococcus
faecalis. Biol. Pharm. Bull. 26(2):266-70.
6) Rahman, S.M., Huda M.N. Uddin, M.N., and Akhteruzzaman,
S. 2002. Short-term administration of conjugated linoleic acid reduces
liver triglyceride concentration and phosphatidate phosphohydrolase
activity in OLETF rats. J. Biochem. Mol. Biol., 35(5): 494-497.
7) Chen. J., Morita. Y., Huda, M. N., Kuroda, T., Mizushima,
T., and Tsuchiya.T. 2002. VmrA, member of a novel class of Na+-coupled
multidrug efflux pump from Vibrio parahaemolyticus. J. Bacteriol.,
184(2):572-576.
8) Huda M. N., Morita, Y. Kuroda, Y., Mizushima, T., and Tsuchiya,
T. 2001. Na+-driven multidrug efflux pump VcaM from Vibrio cholerae
non-01, a non-halophilic bacterium. FEMS Microbiol. Letts, 203: 235-239.
9) Kaneko, J., Mascarenas, A. L., Huda, M. N., Tomita, T.,
and kamio, Y. 1998. An N-terminal region of LukF of Staphylococcal
Leukocidin/gama-hemolysis crucial for the biological activity of the
toxin. Biosci. Biotechnol. Biochem. 62(7): 1465-1467.
10) Huda, M.N., Rahman, S.M., Shekhar, H.U., and Hawlader,
Z.H. 1997. Optimization of a feeding solution for Hirudo menilensis.
Bangladesh J. Zool., 25(1): 89-90.
11) Uddin, M.A., Huda, M.N., and Khan, N.H. 1996. High-performance
liquid chromatographic detection of Diosbulbin D and 8-Epidiosbulbin
E acetate in Dioscorea bulbifera tubers. Bangladesh J. Biochem., 2(2):
95-101.
12) Shekhar, H.U., Alam, M.N., Hawlader, Z.H., Huda, M.N.,
and Hossain, A. 1996. Storage effect on the physicochemical properties
of soyabean oil. Bangladesh J. Biochem., 2(1): 41-48.
13) Huda, M.N., Rahman, M., Khan, N.H. 1995. Natural fermentation
and nutritive values of Lens esculenta L. Dhaka Univ. J. Biol. Sci.,
4(2): 99-106.
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